Karyology study on Bleak (Alburnus alburnus) from the South Caspian Sea region

Karyology study on Bleak (Alburnus alburnus) from the South Caspian Sea region

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The chromosomal spread and karyotype of Bleak (Alburnus alburnus) from Anzali lagoon were identified using tissue squashing techniques with injection of 0.5ml/100g body weight of 0.01% Colchicines solution in fish fingerlings.

Kidney and gill tissues were then extracted and chopped in KCl 0.045M for 20 12n/1200 wella min and fixed in Carnoy solution in 3 stages.The chromosomal spreads were stained in 20% Gimsa for 30 min.

From 347 chromosomal spread counts, the results showed diploid chromosome number of this species 2n=50.Karyotype sten jacket m composed of 7 metacentric, 13 submetacentric and 5 acrocentric or subtelocentric choromosome pairs, and the number of chromosome arms (NF) was determined as NF=90.